An analysis of adenovirus strains isolated in the Soviet Union from 1976 to 1988 revealed four genome types of adenovirus type 3 (Ad3), i.e., Ad3a4, Ad3a9, Ad3a10, and Ad3a11, and four genome types of adenovirus type 7 (Ad7), i.e., Ad7p, Ad7a, Ad7a(1-5), and Ad7f1, identified with the DNA restriction enzymes BamHI, BglII, and HindIII. Three of them, Ad3a10, Ad3a11, and Ad7f1, are newly discovered. The genetic heterogeneity of adenoviruses was examined with restriction endonuclease Cfr13I with a 4-base recognition cleavage site. Eighteen different restriction patterns were identified among 21 selected Ad3 strains after cleavage of DNA with Cfr13I. Eight different subtypes were identified among 20 Ad7 strains by the same technique. For estimation of the relationships among these genome subtypes, pairwise analyses of comigrating DNA restriction fragments from isolates of Ad3 and Ad7 were done after digestion with Cfr13I or with restriction endonucleases recognizing DNA sequences of 6 bp. Surprisingly, the results were very discrepant.
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机译：1976年至1988年在苏联分离出的腺病毒株的分析揭示了腺病毒3型（Ad3）的四种基因组类型，即Ad3a4，Ad3a9，Ad3a10和Ad3a11，以及腺病毒7型的四种基因组类型（Ad7），即，Ad7p，Ad7a，Ad7a（1-5）和Ad7f1用DNA限制酶BamHI，BglII和HindIII鉴定。其中三个是Ad3a10，Ad3a11和Ad7f1。用具有4-碱基识别切割位点的限制性核酸内切酶Cfr13I检查腺病毒的遗传异质性。用Cfr13I切割DNA后，在21个选定的Ad3菌株中鉴定出18种不同的限制模式。通过相同的技术在20个Ad7菌株中鉴定出8个不同的亚型。为了估计这些基因组亚型之间的关系，在用Cfr13I或识别6 bp DNA序列的限制性核酸内切酶消化后，对来自Ad3和Ad7分离株的DNA限制性片段进行配对分析。令人惊讶的是，结果差异很大。
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