We studied the conditions necessary for optimal recovery of bacteria from cerebrospinal fluid. Our results indicated that Streptopcoccus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae can be quantitatively recovered in the sediment after centrifugation at 1,500 X g for 15 min. Equivalent numbers of bacteria were recovered by centrifugation or filtration of antibiotic-free cerebrospinal fluid; however, bacterial recovery by filtration was less effective with antibiotic-supplemented cerebrospinal fluid.
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机译:我们研究了从脑脊液中最佳回收细菌的必要条件。我们的结果表明,在1500 X g离心15分钟后,沉淀物中可定量回收肺炎链球菌,脑膜炎奈瑟氏球菌和流感嗜血杆菌。通过离心或过滤无抗生素的脑脊液回收等效数量的细菌;但是,补充抗生素的脑脊髓液通过过滤回收细菌效果较差。
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