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首页> 外文期刊>Journal of Clinical Microbiology >Genome typing of mouse adenoviruses.
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Genome typing of mouse adenoviruses.

机译:小鼠腺病毒的基因组分型。

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Restriction endonuclease cleavage site analysis was used to differentiate between mouse adenovirus (MAV) types 1 and 2 strains. Viral DNA of suitable purity and quantity for multiple enzymatic digestions was obtained from cloned CMT-93 mouse tumor cells infected with each type of MAV. Clear differences between the MAV-1 (FL) and MAV-2 (K87) genomes were observed after cleavage with restriction enzymes such as BglII, EcoRI, and PaeR7. Fast electrophoresis of DNA fragments in miniature agarose slab gels allowed rapid and unequivocal identification of the MAV strains. This relatively simple and accurate method should be quite useful to determine the different modes of transmission of mouse adenoviruses and their presence in various animal populations.
机译:限制性核酸内切酶切割位点分析用于区分小鼠腺病毒(MAV)1型和2型菌株。从感染了每种类型的MAV的克隆的CMT-93小鼠肿瘤细胞中获得了适合多种酶消化的纯度和数量合适的病毒DNA。用限制酶(例如BglII,EcoRI和PaeR7)切割后,观察到MAV-1(FL)和MAV-2(K87)基因组之间存在明显差异。在微型琼脂糖平板凝胶中对DNA片段进行快速电泳,可以快速,明确地鉴定MAV菌株。这种相对简单和准确的方法对于确定小鼠腺病毒的不同传播方式及其在各种动物种群中的存在应该非常有用。

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