Replication of murine paramyxoviruses in hamster tracheal organ culture and comparison with standard tissue culture methods.

摘要

Replication of Sendai virus, pneumonia virus of mice, and SV5 was investigated in tracheal organ cultures from 2- to 4-day-old and 2- and 4-week-old hamsters, and viral infectivity in tracheal explants was compared with that in tissue culture monolayers. Explants from 2- to 4-day-old hamsters produced higher titers of the three paramyxoviruses, as detected by hemadsorption with guinea pig and murine erythrocytes in primary rhesus monkey kidney cells. Tracheal cultures from 2- and 4-week-old hamsters yielded 1.5 and 2.5 log10 lower infectivity titers. Infected explants exhibited cytopathological changes that correlated well with cessation of ciliary activity. Viral titers in BHK-21, Vero, and BS-C-1 monolayer cells, the systems commonly used for isolation and propagation of murine paramyxoviruses, were lower than those in 2- to 4-day-old hamster tracheal explants. These observations suggest that hamster trachea organ culture could have practical application as an aid for primary isolation of paramyxoviruses from clinical specimens from rodents with respiratory ailments.