Among 51 strains of anaerobic gram-negative cocci belonging to the family Veillonellaceae, all strains of Veillonella (V. parvula and V. alcalescens) displayed red fluorescence under long-wave (366 nm) ultraviolet light, whereas no Acidaminococcus or Megasphaera demonstrated fluorescence. In contrast to Bacteroides melaninogenicus, growth of Veillonella does not require hemin and menadione, and flourescence is rapidly lost upon exposure to air. The fluorescent component of a strain of V. parvula examined could not be extracted in solution with water, ether, methanol, or chloroform, but was readily extracted with 0.4 N NaOH. Spectrophotofluorometrically, the fluorescence maximum of this extract was 660 nm with an excitation maximum of 300 nm, when measured at pH 7.2 and 25 C. Coupled with the Gram stain, ultraviolet fluorescence may be a useful tool for rapid screening of Veillonella and is particularly helpful for detection and, isolation of this organism from mixed culture.
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机译：在属于Veillonellaceae科的51株厌氧革兰氏阴性球菌中，所有Veillonella菌株（细小弧菌和alcalescens）在长波（366 nm）紫外光下均显示红色荧光，而酸氨基球菌或巨球藻均未显示荧光。与黑色素杆菌不同，Veillonella的生长不需要血红素和甲萘醌，并且暴露在空气中会迅速失去荧光。所检查的小肠弧菌菌株的荧光成分不能用水，乙醚，甲醇或氯仿在溶液中提取，但很容易用0.4 N NaOH提取。用分光光度法，当在pH 7.2和25 C下测量时，该提取物的最大荧光值为660 nm，最大激发波长为300 nm。与革兰氏染色相结合，紫外荧光可能是快速筛选Veillonella的有用工具，特别有用用于从混合培养物中检测和分离这种生物。
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