首页> 外文期刊>BMC Genomics >Genome analysis of the sugar beet pathogen Rhizoctonia solani AG2-2IIIB revealed high numbers in secreted proteins and cell wall degrading enzymes
【24h】

Genome analysis of the sugar beet pathogen Rhizoctonia solani AG2-2IIIB revealed high numbers in secreted proteins and cell wall degrading enzymes

机译:对甜菜病原体Rhizoctonia solani AG2-2IIIB的基因组分析表明,分泌的蛋白质和细胞壁降解酶数量很高

获取原文
获取外文期刊封面目录资料

摘要

Background Sugar beet ( Beta vulgaris ) is a crop cultivated for its high content in sugar, but?it is vulnerable to many soil-borne pathogens. One of them is the basidiomycete Rhizoctonia solani . This fungal species has a compatibility system regulating hyphal fusions (anastomosis). Consequently, R. solani species are categorized in anastomosis groups (AGs). AG2-2IIIB isolates are most aggressive on sugar beet. In the present study, we report on the draft genome of R. solani AG2-2IIIB using the Illumina technology. Genome analysis, interpretation and comparative genomics of five sequenced R. solani isolates were carried out. Results The draft genome of R. solani AG2-2IIIB has an estimated size of 56.02?Mb. In addition, two normalized EST libraries were sequenced. In total 20,790 of 21,980 AG2-2IIIB isotigs (transcript isoforms) were mapped on the genome with more than 95?% sequence identity. The genome of R. solani AG2-2IIIB was predicted to harbor 11,897 genes and 4908 were found to be isolate-specific. R. solani AG2-2IIIB was predicted to contain 1142 putatively secreted proteins and 473 of them were found to be unique for this isolate. The R. solani AG2-2IIIB genome encodes a high number of carbohydrate active enzymes. The highest numbers were observed for the polysaccharide lyases family 1 (PL-1), glycoside hydrolase family 43 (GH-43) and carbohydrate estarase family 12 (CE-12). Transcription analysis of selected genes representing different enzyme clades revealed a mixed pattern of up- and down-regulation six days after infection on sugar beets featuring variable levels of resistance compared to mycelia of the fungus grown in vitro. Conclusions The established R. solani AG2-2IIIB genome and EST sequences provide important information on the gene content, gene structure and transcriptional activity for this sugar beet pathogen. The enriched genomic platform provides an important platform to enhance our understanding of R. solani biology.
机译:背景甜菜(Beta vulgaris)是一种因糖分高含量而种植的农作物,但易受许多土壤传播的病原体侵害。其中之一是担子菌Rhizoctonia solani。该真菌物种具有调节菌丝融合(吻合)的相容性系统。因此,茄根红螺菌物种被分类为吻合组(AGs)。 AG2-2IIIB分离株对甜菜最具攻击性。在本研究中,我们报告了使用Illumina技术的茄红细菌AG2-2IIIB的基因组草图。进行了五个测序的R. solani分离株的基因组分析,解释和比较基因组学。结果solani R. solani AG2-2IIIB的基因组草图估计大小为56.02?Mb。另外,对两个标准化的EST文库进行了测序。总共21,980个AG2-2IIIB异构体(转录异构体)中有20,790个以95%以上的序列同一性在基因组上作图。预计R.solani AG2-2IIIB的基因组将包含11,897个基因,并且发现4908个是分离物特异性的。预计R. solani AG2-2IIIB包含1142个假定分泌的蛋白,其中473个对于该分离株而言是独特的。茄红细菌AG2-2IIIB基因组编码大量碳水化合物活性酶。多糖裂解酶家族1(PL-1),糖苷水解酶家族43(GH-43)和碳水化合物Estarase家族12(CE-12)的数量最高。对代表不同酶进化枝的选定基因的转录分析显示,在甜菜上感染后六天,与体外生长的真菌的菌丝体相比,抗性水平变化,上调和下调的混合模式。结论已建立的茄红枯菌AG2-2IIIB基因组和EST序列为该甜菜病原体的基因含量,基因结构和转录活性提供了重要信息。丰富的基因组平台提供了一个重要平台,可增强我们对茄红梭菌生物学的了解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号