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In vitro osteoinductive potential of porous monetite for bone tissue engineering

机译:多孔芒硝在骨组织工程中的体外骨诱导潜力

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Tissue engineering–based bone grafts are emerging as a viable alternative treatment modality to repair and regenerate tissues damaged as a result of disease or injury. The choice of the biomaterial component is a critical determinant of the success of the graft or scaffold; essentially, it must induce and allow native tissue integration, and most importantly mimic the hierarchical structure of the native bone. Calcium phosphate bioceramics are widely used in orthopaedics and dentistry applications due to their similarity to bone mineral and their ability to induce a favourable biological response. One such material is monetite, which is biocompatible, osteoconductive and has the ability to be resorbed under physiological conditions. The osteoinductive properties of monetite in vivo are known; however, little is known of the direct effect on osteoinduction of human mesenchymal stem cells in vitro. In this study, we evaluated the potential of monetite to induce and sustain human mesenchymal stem cells towards osteogenic differentiation. Human mesenchymal stem cells were seeded on the monetite scaffold in the absence of differentiating factors for up to 28 days. The gene expression profile of bone-specific markers in cells on monetite scaffold was compared to the control material hydroxyapatite. At day 14, we observed a marked increase in alkaline phosphatase, osteocalcin and osteonectin expressions. This study provides evidence of a suitable material that has potential properties to be used as a tissue engineering scaffold.
机译:基于组织工程的骨移植物正在成为一种可行的替代治疗方式,以修复和再生由于疾病或伤害而受损的组织。生物材料成分的选择是移植物或支架成功的关键决定因素。本质上,它必须诱导并允许天然组织整合,最重要的是模仿天然骨骼的层次结构。磷酸钙生物陶瓷由于其与骨矿物质的相似性和诱导良好生物反应的能力而被广泛用于骨科和牙科领域。一种这样的材料是透钙石,其具有生物相容性,骨传导性并且具有在生理条件下被吸收的能力。透钙石在体内的骨诱导特性是已知的。然而,对体外对人间充质干细胞骨诱导的直接影响知之甚少。在这项研究中,我们评估了褐铁矿诱导和维持人类间充质干细胞向成骨细胞分化的潜力。在不存在分化因子的情况下,将人间充质干细胞播种在磁铁矿支架上长达28天。比较了在沸石支架上细胞中骨特异性标记物的基因表达谱与对照材料羟磷灰石。在第14天,我们观察到碱性磷酸酶,骨钙素和骨连接素表达明显增加。这项研究提供了合适的材料的证据,该材料具有用作组织工程支架的潜在特性。

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