Dependence of Fluorescence Spectra of Susabi-nori (Porphyra yezoensis) on the Excitation Wavelength

摘要

References(7) This research paper aims to investigate dependence of fluorescence spectra of Susabi-nori (Porphyra yezoensis) on the excitation wavelength by comparing with absorption spectra of photosynthetic pigments. The intensities of the fluorescent peaks located at approximately 685 nm and 720 nm relatively increased by using the excitation light with the wavelength of approximately 430 nm, because the excitation light was mostly absorbed by chlorophyll-a. On the other hand, the intensity of the 580 nm peak relatively increased by using that of approximately 500 nm, because the excitation light was mostly absorbed by phycoerythrin. As a result, the intensity ratio of the 580 nm peak to the 685 nm peak was maximal at the excitation wavelength of approximately 500 nm. Therefore, it was concluded that the optimum excitation wavelength was approximately 500 nm for diagnosis of diseases and stresses of Susabi-nori.