Fucoxanthin extraction and fatty acid analysis of Sargassum binderi and S. duplicatum

摘要

Fucoxanthin has been successfully extracted and purified from two species of Malaysian brown seaweeds, namely?S. binderi?and?S. duplicatum.?The purity of the fucoxanthin is >99% as indicated by HPLC analysis. Fucoxanthin content, total lipid and fatty acid composition of the seaweeds showed that both samples contained a considerable amount of fucoxanthin and total lipid. The amount of fucoxanthin and total lipid contents of?S. duplicatum?(1.01 ± 0.10 and 21.3 ± 0.10 mg/g dry-weight, respectively) was significantly higher than those of?S. binderi?(0.73 ± 0.39 and16.6 ± 4.10, respectively). Both types of seaweeds also contained a considerable amount of unsaturated fatty acids. However, in terms of docosahexanoic acid, eicosapentanoic acid, arachidonic acid, linoleic acid and alpha-linolenic acid contents,?S. duplicatum?was found to be higher (0.76, 2.55, 13.64, 5.81 and 5.35%, respectively) than?S. binderi?(0.70, 1.82, 9.13, 6.37 and 4.39%, respectively).? For saturated fatty acids, palmitic (C16:0) was found to be the major fatty acid in both samples studied.