Analysis of polymorphisms of mitochondrial DNA D-loop and Mc1R gene in Chinese Wuchuan Black cattle

摘要

Chinese Wuchuan Black cattle is an important indigenous breed in Guizhou province, China. However, little is known about the genetic characterization and origin of this breed. In the present study, the polymorphism and variation of complete mitochondrial DNA D-loop sequences were analyzed. Fifty-one nucleotide polymorphic sites were detected; the nucleotide diversity ( ???? ) was 5.475%. Eleven haplotypes were determined (accession numbers: HM106460?￠????HM106470) by biological software with a haplotype diversity ( h ) of 0.909. This suggests that plenty of genetic diversity exists in mtDNA D-loop region in Chinese Wuchuan Black cattle. A phylogenetic tree based on mtDNA D-loop sequences suggested that the origin of Chinese Wuchuan Black cattle was affected by Bos taurus and Bos indicus . In addition, coding sequence (CDS) regions of Mc1R gene for this breed were sequenced. Six polymorphic sites were detected with out insertions and deletions, only one transversion was observed. A novel single nucleotide polymorphism (SNP;[T/C]) at the position of 663 bp of Mc1R gene was detected in this cattle breed. This suggests that the novel SNP (T/C) might be used as a specific molecular marker to aid for breed identification.