Evaluation of the Antinociceptive Effect of Hydroalcoholic Extract of Potentilla Reptans L. in the Adult Male Rat

摘要

Introduction: Medicinal plants have been a focus of attention to researchers due to their fewer side effects in treating diseases comared to chemical drugs. Some health properties of medicinal plants such as anti-hypercholesterolemia and antioxidant effects of Potentilla reptans have been proven. Therefore, the present study aimed to investigate the antinociceptive and analgesic effect of hydro-alcoholic extract of Potentilla reptans L. (HEP) in the male rat.Methods: In this experimental study, 36 male rats were divided into 6 groups, including: control group (normal saline), morphine group (1mg/kg), HEP groups (treated with 25, 50 and 100 mg/kg), and naloxone group (200 mg/kg). Writhing, formalin and tail-flick tests were used to evaluate the analgesic effects of the extract. In order to analyze the study data, variance and Tukey test were applied.Results: The results of the present study demonstrated that in writhing test (P0.05 and P0.001 respectively), formalin test (P0.05, P0.001) and tail-flick test (P0.05, P0.01); the injection of 50, and 100mg/kg dose of the extract produced significant analgesic effects compared with the control group.  50mg/kg dose of Naloxone injection extract has shown a significant analgesic effect in the formalin test (P0.05). In addition, the effect of the analgesic dose of 100mg/kg of the extract in formalin test was similar to that of morphine.Conclusions: The findings of the current study revealed that Potentilla reptans L. (HEP) has a significant analgesic effect. Opioid pathway seems to be one of the possible mechanisms of extract effects, which further clinical trials are recommended  in this case.