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Extraction of ancestral constituents of natural polyploids

机译:天然多倍体祖先成分的提取

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摘要

Pentaploids (2n = 5x = 35, AABBD) and 34-chromosome plants, obtained after the first backcross, were backcrossed to 4 varieties (Chinese Spring, Peko, Koga and Svenno) and 11 monosomic (2n = 41) lines of Triticum aestivum (2n = 6x = 42, AABBDD). The seed sets with the 4 varieties of Triticum aestivum ranged from 0.6 % to 12 %. The average seed set (8 %) in the second backcross was less than the average seed set (11 %) obtained from the first backcross. The percentage of the seed set (10 %) with Chinese Spring disomics was more than double that obtained with the monosomics (4 %). Monosomics 6 B and 3 D were more successful (4 % seed set) than monosomics 1 D and 4 D (2 % seed set). The seed set in the pentaploids (AABBD) varied with the original tetraploid (AABB) parent. An advantage of the general backcross appears to be that it may replace and balance certain disharmonious combinations that occur in aestivum X durum segregants. Since one of the aims of extraction is to analyse the genetic diploidization of allopolyploids, it is important to use only those species and varieties which have been isolated from introgression from related taxa.
机译:在第一次回交后获得的五倍体(2n = 5x = 35,AABBD)和34染色体植物回交到4个品种(中国春,Peko,Koga和Svenno)和11个单粒(2n = 41)小麦(Triticum aestivum)品系( 2n = 6x = 42,AABBDD)。普通小麦的4个变种的种子集范围为0.6%至12%。第二次回交的平均结实率(8%)小于第一次回交获得的平均结实率(11%)。中国春季二体组学的结实率(10%)是单体组学(4%)的两倍以上。单体组6 B和3 D比单体组1 D和4 D(种子组2%)更成功(种子定型4%)。五倍体(AABBD)中的种子与原始四倍体(AABB)亲本不同。一般回交的一个优点似乎是,它可以替代和平衡在普通小麦X硬粒小麦分离子中发生的某些不和谐组合。由于提取的目的之一是分析同素多倍体的遗传二倍体,重要的是仅使用那些从相关分类群的渗入中分离出来的物种和品种。

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