Two‐dimensional gel electrophoresis of dog plasma proteins: Genetic polymorphism of an α1‐protease inhibitor and another postalbumin

摘要

Two-dimensional electrophoretic analysis of dog plasma proteins was done by a first-dimension separation in agarose gel (pH 5.4) followed by a second-dimension separation in horizontal poly-acrylamide gel (pH 9.0). Two groups of α1-globulins, designated Pi-1 and Pi-2, were found to be protease inhibitors. Extensive genetic polymorphism of Pi-1 and of another unidentified α-globulin, tentatively designated postalbumin (Pa), was observed. Family data supported the hypothesis that Pi-1 and Pa polymorphisms were controlled by codominant, autosomal alleles. Three Pi-1 alleles and two Pa alleles were observed in 60 breeds (1119 dogs) studied. Each of the Pi-1 alleles gave rise to a single strong fraction while each of the Pa alleles showed a major and a minor anodic fraction. The present study, along with earlier reports, showed that some of the proteins and enzymes (albumin, transferrin, eserine resistant esterase, α1-protease inhibitor and postalbumin) in plasma exhibit considerably more polymorphism than that reported for hemoglobin and some of the red cell and tissue enzymes of the domestic dog.