The Effects of Cellular Seeding Density on Microencapsulated Recombinant Cho Cells Growth, Metabolism and Production of Endostatin

摘要

Microencapsulation technology is an alternative large-scale mammalian cells culture method. The semi-permeable membrane of microcapsule allows free diffusion of nutrients, oxygen and toxic metabolites, provides a mild and comfortable environment for cell growth and it can also protect cells from mechanical damage of shear forces associated with agitation and aeration; therefore the microencapsulated cells culture favor to long-term growth of cells and can obtain higher cell density. The cellular seeding density is important control parameter in mammalian cell culture _Cit directly effects cell growth, metabolism and protein production. Optimization of the cellular seeding density is very important for establishing ideal culture conditions. The aim of this study was to assess the effects of different cellular seeding density on microencapsulated recombinant CHO cell growth, metabolism and endostatin production and optimize the cellular seeding density. The results showed that the maximal cell density and yield of endostatin reached 1.91x10 7 cells mL -1 microcapsule and 615.1ng mL -1 during the culture when the cellular seeding density was 3x10 6 cells mL -1 microcapsule. The metabolism behavior of glucose and glutamine shifted as the seeding density was altered. The energy metabolism was more efficient as the cellular seeding density was at a lower level, more glucose and glutamine were utilized for biosynthesis and less lactate and ammonium were produced.