In-vitro interactions of Ciprofloxacin Hydrochloride with different essential mineral salts and its influence on antimicrobial activity (MIC) of Ciprofloxacin Hydrochloride

摘要

Present work describes the interactions of Ciprofloxacin Hydrochloride with different essential mineral salt like Magnesium Sulfate, Manganese Sulfate, Ferrous Sulfate, Zinc Sulfate and Potassium Chloride in an aqueous system at pH 7.4. This Magnesium Sulfate, Manganese Sulfate, Ferrous Sulfate, Zinc Sulfate and Potassium Chloride are essential trace element. From spectrophotometric study, it has been found that Ciprofloxacin Hydrochloride forms 1:1 complex with Magnesium Sulfate, Manganese Sulfate, Ferrous Sulfate, Zinc Sulfate and Potassium Chloride. Spectral studies helps to detect the initial complexation between drug and mineral salts. Job’s plot at 7.4 provides same type of information. An individual antimicrobial study (MIC) of Ciprofloxacin Hydrochloride in 1:1 mixture with Magnesium Sulfate, Manganese Sulfate, Ferrous Sulfate, Zinc Sulfate and Potassium Chloride at pH 7.4 was performed. These studies were carried out by observing the minimum inhibitory concentration (MIC) of the complexes and compared with the parent Cephradine against both Gram negative and Gram positive microorganisms in nutrient broth medium. Study confirms interactions of the Cephradine with Magnesium Sulfate, Manganese Sulfate, Ferrous Sulfate, Zinc Sulfate and Potassium Chloride and the interactions results into change the antimicrobial activity of Cephradine. Result shows that the antimicrobial activity increasing trends in presence of Magnesium Sulfate, Manganese Sulfate, Ferrous Sulfate and decreasing trends in presence of Zinc Sulfate and Potassium Chloride. DOI: http://dx.doi.org/10.3329/ijpls.v1i3.12977 International Journal of Pharmaceutical and Life Sciences Vol.1(3) 2012