In Vitro Bioactivity and MG63 Cytocompatibility of Chitosan-Silicate Hybrids

摘要

This paper describes in vitro bioactivity and cytocompatibility of chitosan-silicate hybrids derived from chitosan?and ?γ-glycidoxypropyltrimethoxysilane (GPTMS) through sol-gel processing. Chitosan, one of the biodegradable polymers, was selected as a hybrid matrix whereas GPTMS was employed as crosslinking agent as well as bioactive inorganic species. GPTMS was hydrolyzed completely in acetic-acid solution to yield Si-OH groups, which in turn were condensed to form Si-O-Si networks. Cytotoxicity of GPTMS monomer was much lower than that of glutaraldehyde. From MG63 osteoblastic cell culture, the chitosan-GPTMS hybrids were cytocompatible better than the hybrids crosslinked with glutaraldehyde, tetraethoxysilane or glycidylmethaacrylate. The hybrids incorporated with Ca ions deposited apatite on their surface under a simulated body fluid (SBF, Kokubo solution). Freeze-drying lead to porous hybrids with about 90% in porosity and about 100 μm in size. When soaked in SBF, the porous deposited apatite not only on the outer surface but also on the pore wall. The porous hybrids hence are considered to be appropriate materials for scaffolds.