Silk Fibroin/Calcium Phosphate Silicate Composites: In vitro Bioactivity

摘要

Composite materials of Silk Fibroin (SF) and Calcium Phosphate Silicate (CPS) ceramic in the 15CaO-6SiO2-0.5P2O5 system were prepared by method of mixing different percentage of component weight. The structure was characterized before and after in vitro test by FTIR, SEM. The concentration of Ca, Si and P after in vitro test was analysed by ICP-AES. FTIR of the composites before in vitro test depicts that random coil and β-sheet structures co-exist in the composites. CPS ceramic has a significant effect on the secondary structure of SF. SEM observed that CPS particles uniformly dispersed in SF matrix. FTIR of the composites after in vitro test revealed that carbonate containing hydroxyapatite (CO3HA) was formed on the surface. B-type CO3HA preferentially formed in the sample with 75 wt. % CPS ceramic. SEM proved that hydroxyapatite (HA) formed in a high level. ICP-AES showed that HA precipitation were similar for the three composites. Based of these results, it can be concluded that the incorporation of CPS ceramic powder enhanced the in vitro bioactivity.