Identification of amplified fragment length polymorphism (AFLP) fragments linked to soybean mosaic virus resistance gene in Glycine soja and conversion to a sequence characterized amplified regions (SCAR) marker for rapid selection

摘要

Soybean mosaic virus (SMV) is a prevalent pathogen that cause yield losses and reduce seed quality in soybean production areas worldwide.?Glycine soja?is the wild ancestor of domesticated soybean and a potential source of new SMV resistance genes (Rsv). In this study, we applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to identify molecular markers linked to the?Rsv?gene in?G. soja?for resistance to SMV strain N3 (the most virulent strain) from Northeast China, then a population of 207 F2?individuals derived from the cross of the SMV resistant line SRY8 (G. soja) with the susceptible line Hefeng25 (Glycine max) for linkage analysis. One dominant AFLP marker, P36M65, was mapped to a position 5.6 cM from?Rsv?and specific polymerase chain reaction (PCR) primers were designed to convert P36M65 into sequence characterized amplified region (SCAR) maker. This new marker may be useful for marker-assisted breeding of RSV in?G. soja.