Antioxidant activity and acute toxicity of Neoglaziovia variegata (Bromeliaceae)

摘要

Antioxidant activities of?Neoglaziovia variegata?were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH)?radical scavenging and β-carotene-linoleic acid bleaching and was compared with ascorbic acid,?butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The total phenolics content of the extracts was determined by the Folin-Ciocalteu method. Total flavonoid was also determined. The most significant total phenolic content was of 543.50 ± 9.38 mg of gallic acid equivalent/g for ethyl acetate extract (AcOEt), which presented the best antioxidant activity (IC50?5.08 ± 0.20 μg/ml) for DPPH scavenging. The acute toxicity of Nv-EtOH was performed 2.0 g/kg intraperitoneally and 5.0 g/kg orally in mice. No mortality and no toxicity signs were observed, indicating low toxicity of the extract. Blood was removed after 14 days for laboratory analysis of hematological and biochemical parameters. Alterations ofaspartate aminotransferase?(AST) and creatinine were observed. The data obtained showed that the doses induced microscopic alterations in the liver and kidney. In conclusion, the Nv-EtOH can be considered of low toxicity.