Comprehensive evaluation of pharmacological properties of Olea europaea L. for Cosmeceuticals prospects

摘要

Propionibacterium acnes (anaerobic bacteria) and Staphylococcus epidermidis (aerobic bacteria) have been acknowledged as key comedone forming pathological factor, eliciting an inflammation in acne. The present study was conducted to evaluate antibacterial and antioxidant activities of Olea europaea leaves extracts (OLE) of different solvents (methanol, ethanol, deionized water, and acetone) against etiologic pathogens of acne vulgaris . Methods The antibacterial testing against the selected pathogen viz ., P. acnes and S. epidermidis were evaluated using broth micro dilution method recommended by CLSI, in duplicate. Correspondingly the total phenolic content and flavonoid content along with radicals scavenging activity by DPPH assay were also evaluated. The data of antibacterial assay demonstrated that these plant extracts differ quantitatively in their activity against the tested pathogens. Results The results (mg/ml) exhibited that Olea europaea leaves extracts (MIC:2.263/IC₅₀:1.626, MIC:0.933/IC₅₀:0.636, MIC:1.054/IC₅₀:1.040, MIC:2.534/IC₅₀:2.500 of aqueous, methanol, ethanol, acetone extracts respectively) are more effective against growth of P.acnes as compared to S. epidermidis (MIC: Range (Not active at particular concentration), IC₅₀: Range, MIC:1.031, IC₅₀: 0.670, MIC:1.502, IC₅₀:1.234, MIC: Range, IC₅₀:1.890?mg/ml aqueous, methanol, ethanol, acetone extracts respectively). The readings were statically analyzed and also compared with standard drug tetracycline. Conclusions The current findings suggested Olea europaea L. as a promising source of potential antioxidants and antibacterial activity against P.acnes and S. epidermidis that may be an efficient therapeutic agent in the pathogenesis of Acne vulgaris and proves a potential source of Cosmeceuticals.