Photodamage attenuation effect by angelic acid in UVA irradiation-induced damages in normal human dermal fibroblast

摘要

Among various extra stimuli of humans, ultraviolet (UV) has been the most studied factor because it arouses not only internal but also external irritation in the body. UVA, one type of UV rays, has a wavelength between 320 and 400 nm and capacity to penetrate the skin dermal layer. Therefore, studies on how to reduce UVA-induced maleficence have been investigated vibrantly. Angelic acid has been demonstrated to aid in wound healing and exhibited sedative and psychotropic properties. But there have not been sufficient reports whether angelic acid has potential properties in the cosmeceutical aspect. To investigate protective effects of angelic acid on UVA-induced oxidative stress and disruption of extracellular matrix, researchers analyzed cell proliferation rate, intracellular reactive oxygen species (ROS) scavenging capacity, cellular senescence, transcriptional activity of activating protein-1 (AP-1) transcription factor, and gene expression of antioxidant enzymes and connective tissue-related proteins. Pretreatment of angelic acid in normal human dermal fibroblasts (NHDFs) showed protective effect on UVA-induced proliferative inhibition. Via estimating ROS scavenging activity, angelic acid represented a scavenging effect of excessive increased intracellular ROS which is induced by UVA irradiation. Through quantitative real-time polymerase reaction, antioxidant enzyme and extracellular matrix (ECM)-related protein coded gene expressions were analyzed. Analysis of senescent cell and AP-1 promoter activity by beta-galactosidase assay and luciferase reporter gene assay, respectively, indicated how angelic acid regulates cellular mechanisms associated with connective tissue density. Through the present study, researchers verify that angelic acid has dermal protective effect against UVA and suggest angelic acid as an efficacious cosmetic material preventing dermal cellular damages.