Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection

摘要

AIM To identify the property of dendritic cells (DCs) of pripheral blood monocytes (PBMC) in patients with chronic HBV infection. METHODS Twenty patients with persistent HBV infection Were included in this study, 10 healthy subjects being Used as a control group. The peripheral blood Mononuclear cells (PBMC) of T cell-depleted populations Were incubated and induced into mature dendritic cells in The RPMI-1640 medium in the presence of cytokines GM- CSF, IL-4, FLt-3, TNF-α and 100 ML. L~-1 of fetal calf serum For a total of 10-12 days.