ANTITUMOR MECHANISM-OF GEM 10 BY THE NATURAL KILLER ACTIVITY AND INTERLEUKIN-2 PRODUCTION

摘要

Objective To investigate the anti-tumor effects of GeM10 by the natural killer(NK) cells activities and the production of Interleukin-2 (IL-2) in peripheral blood mononuclear cells (PBMNCs). Methods Assay of human NK cells activities by dye rejection assay in vitro and production of IL-2 in PBMNC by IL-2 bioassay with IL-2 dependent cell line CTLL2 and MTT colorometric method. Results GeM10 could significantly stimulate NK activities (60,mug centre dot Ml~(-1) GeM10: 17.077 + - 7.665, 120 MU g centre dot mL~1 GeM10: 24.9 + - 13.04; control: 7.72 + - 4.64, P<0. 05). GeM10 could up-regulate the production of IL-2 of PBMNCs in tumor patients(60 mu g centre dot mL~1 GeM10: 2. 965 + - 1.183; 120 mu g- mL~(-1) GeM10; 2.28±0.847; control: 1.792 + 0.823, P<0. 05). Conclusion The GeM10 not only can stimulate the NK activities but also increase the IL-2 production by PBMNCs in tumor patients. These findings indicate that the GeM10 may have promise as an anti-tumor drug and a biological response modifier in clinic.