Chondrocyte Apoptosis in Articular Cartilage with Kashin-Beck Disease

摘要

To investigate the apoptosis of articular chondrocyte and the expression of BcI-2, Bax, Fas and iNos in articular cartilage with Kastain-Beck disease (KBD) in order to understand the pathogenesis of chondronecrosis in KBD. Methods The collected samples of human articular cartilage were divided into two groups: control group (15 samples from 15 cases) , KBD group (15 samples from 15 cases). KBD patients were diagnosed by "Pathological Criteria to Diagnose KBD in China". Chondrocyte apoptosis was detected by TUNEL staining, and the Bcl-2, Bax, Fas and iNos positive articular chondrocytes were stained by the B-SA of immunohistochemistry. Articular cartilage was classified three zones and the positive rate were counted by light microscopes for cytoplasimic staining by polyclonal antibodies of Bcl-2, Bax, Fas and iNos and apoptotic chondrocytes by TUNEL. Results 1 The percentage of positive apoptotic chondrocytes stained by TUNEL in the middle zone of articular cartilage from the KBD-children group(33.60 + 2.71 percent) was higher than that of the control (1.33 + 0.41 percent (=11.59, g=28, P<0. 01). 2The percentage of chondrocytes staining for Bcl-2, Bax, Fas and iNos among the upper and the middle zone in KBD group were significantly higher than that of the control ((=11.75-18.65, g= 14, P<0. 01); the remarkable difference in the expression of Bcl-2, Bax, Fas and iNos among the upper, the middle and the deep zones was also seen in KBD articular cartilage (F=73. 49 - 114. 42, g=42, P<0. 01), and staining for Bcl-2, Bax, Fas and iNos in KBD children was prominent in the upper zone (41. 93+12. 26 percent, 45.60 + 15.78 percent, 53.60 + 16.49 percent, 45.47 + 14.02 percent) and the middle zone(14. 93 + 3. 50 percent , 13. 87 + 4. 32 percent , 23. 27 + 4. 83 percent , 21. 67 + 6. 82 percent) of articular cartilage, respectively. Conclusion The chondrocyte apoptosis and the present of Bcl-2, Bax, Fas and iNos positive chondrocytes in articular cartilage of children with KBD were significantly higher than that of the control.