Long noncoding RNA AC092171.4 promotes hepatocellular carcinoma progression by sponging microRNA-1271 and upregulating GRB2

摘要

In this study, we investigated the mechanistic role of the long non-coding RNA (lncRNA) in hepatocellular carcinoma (HCC). was significantly upregulated in HCC tumor tissues compared to normal liver tissues. HCC patients with high expression showed poorer overall survival (OS) and disease-free survival (DFS) than those with low expression. cell proliferation, migration and invasiveness were all higher in -overexpressing HCC cells, but lower in -silenced HCC cells, than in controls. Balb/c nude mice injected with -silenced HCC cells had smaller xenograft tumors, which showed less growth and pulmonary metastasis than control tumors. Bioinformatics analyses and dual luciferase reporter assays confirmed that binds directly to miR-1271, which targets the 3’UTR of GRB2 mRNA. expression correlates negatively with miR1271 expression and correlates positively with GRB2 mRNA expression in HCC tissues from patients. HCC cells co-transfected with miR-1271 mimics and sh- show less proliferation, migration, invasiveness, GRB2 protein, and epithelial to mesencyhmal transition (EMT) than sh- -transfected HCC cells. These findings demonstrate that promotes growth and progression of HCC by sponging miR-1271 and upregulating GRB2. This makes a potential prognostic indicator and therapeutic target for HCC patients.