miR-380-3p regulates melanogenesis by targeting SOX6 in melanocytes from alpacas (Vicugna pacos)

摘要

miR-380-3p and SOX6 expression in alpaca skin samples with different hair colors and in melanocytes. RT-qPCR analysis of miR-380-3p expression in alpaca skin samples with different hair colors. RT-qPCR analysis of SOX6 mRNA expression in alpaca skin samples with different hair colors. Western blot analysis of SOX6 protein expression in alpaca skin samples with different hair colors. Quantitative analysis of SOX6 protein expression in alpaca skin samples with different hair colors. Localization of miR-380-3p in alpaca melanocytes in vitro by in situ hybridization analysis. miRNA, mRNA and protein expression levels were normalized to those of U6 mRNA, 18S rRNA and β-actin, respectively. The bars represent the mean ± standard error (  = 3). *  P P