Effect of a passive sonic irrigation system on elimination of Enterococcus faecalis from root canal systems of primary teeth using different concentrations of sodium hypochlorite: An in vitro evaluation

摘要

>Background. This in vitro study aimed to compare the antibacterial effect of different concentrations of sodium hypochlorite on elimination of Enterococcus faecalis from root canal systems of primary teeth with or without a passive sonic irrigation system (EndoActivator). >Methods. The root canals of 120 extracted single-rooted primary incisors were prepared using the crown-down technique. The teeth were autoclaved and inoculated with E. faecalis. The infected samples were then randomly divided into 6 experimental groups of 15 and positive and negative control groups as follows: group 1: 0.5% sodium hypochlorite solution; group 2: 2.5% sodium hypochlorite solution; group 3: 5% sodium hypochlorite solution; group 4: 0.5% sodium hypochlorite solution + sonic activation; group 5: 2.5% sodium hypochlorite solution + sonic activation; and group 6: 5% sodium hypochlorite solution + sonic activation. Microbiological samples were collected before and after disinfection procedures and the colony-forming units were counted. Statistical analyseswere performed using the two-way ANOVA and post hoc Duncan's tests in casesof significant difference.>Results. There were no significant differences between thegroups in any of the variables (concentration of antiseptic or use ofsonic irrigation system).>Conclusion. Use of passive sonic irrigation systems inendodontic treatment of single-rooted primary teeth is of no benefit comparedto regular needle irrigation. The results of this study also recommends useof lower concentrations of sodium hypochlorite solution (0.5%) for irrigationof the root canal system rather than higher concentrations givenapproximately equal efficacy.