新疆核桃根际土壤中解磷菌的分离筛选及鉴定

摘要

In order to provide strain material for development of specific walnut microbial fertilizer, through separation and purification of the phosphate-solubilizing bacteria in rhizosphere soil ofXinjiang walnut, some high quality and efficient phosphate-solubilizing strains were screened, which had growth-promoting effect for walnut tree and could colonize stably in its rhizosphere. 54 strains of phosphate-solubilizing bacteria were isolated from the rhizosphere soil of Aksu, Hotan and Kashgar walnut-producing areas in Xinjiang, ability of its phosphate-solubilizing were measured by using the methods of dissolved phosphorus ring screening and liquid shakeflask screening, and 14 strains of bacteria with strong phosphate-solubilizing ability were screened out. 13 strains of phosphate-solubilizing bacteria were reserved after inducted by rifampicin resistance, and eventually 11 strains of phosphate-solubilizing bacteria were selected out after the field colonization experiments. The11 strains of phosphate-solubilizing bacteria were identified by 16S rDNA, they belonged to 5 genera, and they werePseudomonas,Staphylococcus, Planomicrobium,Microbacterium, and Acinetobacter. Among them,Pseudomonas was the dominant population and its phosphate-solubilizing effect was best.%为给核桃专用微生物肥的研制提供菌株材料,通过对新疆核桃根际土壤中解磷菌的分离纯化,筛选出对核桃树具有促生作用且能在其根际稳定定殖的优质高效解磷菌株.从阿克苏、和田、喀什等新疆核桃主产区的根际土壤中分离出解磷菌54株,采用溶磷圈初筛法和液体摇瓶复筛法对其解磷能力进行测定,筛选出解磷能力强的菌株14株.经耐利福平诱导后剩余13株,再进行大田定殖试验,最终筛选出11株解磷菌.经16S rDNA鉴定,得出这11株解磷菌归属于5个属,分别为假单胞菌属Pseudomonas,葡萄球菌属Staphylococcus,动性杆菌属Planomicrobium,微杆菌属Microbacterium,不动杆菌属Acinetobacter,其中,假单胞菌属为优势种群且解磷效果最好.