Development and Characterization of SYBR Green I Based RT-PCR Assay for Detection of Omsk Hemorrhagic Fever Virus

摘要

Dear Editor,Omsk hemorrhagic fever virus(OHFV)belongs to the tickborne virus group within Flavivirus,in Flaviviridae family(Holbrook et al.2005)which consists of several important human pathogens including tick-borne encephalitis virus(TBEV),Powassan virus(POWV),Louping ill virus(LIV),Langat virus(LGTV)and Kyasnaur Forest disease virus(KFDV)(Yoshii and Holbrook 2009).OHFV is an enveloped virus containing a single-stranded positive-sense RNA genome of approximately 11,000 nucleotides.The genome has a single open reading frame,which encodes three structural proteins[capsid(C),pre-membrane(prM)or membrane(M),and envelop(E)]and seven non-structural proteins(NS1,NS2a,NS2b,NS3,NS4a,NS4b and NS5)between the 5'untranslated region(UTR)and 3'UTR(Chambers et al.1990).