龙脑樟幼茎愈伤组织的诱导

摘要

以龙脑樟Cinnamomum camphorachvar. Borneol组培苗幼茎为材料诱导优良愈伤组织，采用正交设计法，以愈伤组织的出愈时间、诱导率、生长状态、鲜重生长指数和右旋龙脑含量为指标，筛选最适培养基，尤其是培养基中最佳生长调节剂组合，为利用龙脑樟细胞悬浮培养调控右旋龙脑提供理论依据。结果表明，龙脑樟组培苗幼茎诱导愈伤组织的最适培养基为MS +2,4-D 2 mg·L-1+6-BA 0.6 mg·L-1，右旋龙脑的提取产量为2.6698μg·g -1 DW。%In this study, caulicle of borneol camphor was used as culture material, the optimal medium with different combination of growth regulator was screened using orthogonal method. The time of callus induction, the growth state, fresh weight growth index and D-borneol content of callus were measured. The results showed that the optimal growth regulator combination was 2 mg·L-1 2,4-D and 0.6 mg·L-1 6-BA, and extract yield of D-borneol was 2.6698μg·g -1 DW.