基于高通量测序分析大花杓兰根际土壤细菌多样性

摘要

[目的]从分子水平上对珍稀濒危药用植物大花杓兰根际土壤细菌群落多样性进行初步探讨.[方法]通过Illumina Miseq高通量测序技术对大花杓兰根际土壤细菌16S rRNA基因的V3～V4高变区片段进行测序,而后进行生物信息学分析.[结果]共得到166,847条16S rRNA基因序列.基于≥97％的相似度水平,通过聚类共获得1549个可操作分类单元(operational taxonomicunits,OTUs).大花杓兰根际土壤细菌在门、纲、目、科和属分类水平上的优势类群分别为变形菌门Proteobacteria (39.67％)、α-变形菌纲α-Proteobacteria (24.72％)、根瘤菌目Rhizobiales(11.11％)、鞘脂单胞菌科Sphingomonadaceae(7.13％)和鞘氨醇单胞菌属Sphingomonas (6.27％).[结论]首次揭示了大花杓兰根际土壤细菌群落的多样性,为进一步探究大花杓兰与其根际细菌的相互关系以及大花杓兰的迁地保育奠定了基础.%[Objective] Rhizospheric bacteria diversity of medicinal plants Cypripedium macranthum endangered was preliminary explored at the molecular level.[Method] V3-V4 variable regions of 16S rRNA gene in C.macranthum rhizospheric soil were sequenced with the Illumina Miseq high throughput sequencing platform,and bioinformatics analysis of these sequences were then performed.[Result] A total of 166,847 partial 16S rRNA gene sequences were obtained.At the 97 ％ sequence similarity levels,1549 operational taxonomic units (OTUs) were identified based on UCLUST algorithm.At the classification level of phylum,class,order,family and genera,the dominant groups of C.macranthum rhizospheric bacteria were Proteobacteria (39.67 ％),α-Proteobacteria (24.72 ％),Rhizobiales (11.11％),Sphingomonadaceae (7.13 ％),Sphingomonas (6.27 ％),respectively.[Conclusion]The research revealed for the first time bacteria diversity in C.macranthum rhizospheric soil.The result of the study would be beneficial for further exploring the interaction between C.macranthum and its rhizospheric bacteria and other related scientific questions.