β-TCP／rhBMP-2复合物的制备及其诱导成骨活性观察

摘要

为了检测β-磷酸三钙／人重组骨形态发生蛋白-2（β-TCP／rhBMP-2）复合物的骨诱导能力，将自制β-TCP／rhBMP-2复合物植入45只小鼠一侧股后肌袋作为实验组，单纯β-TCP植入对侧作为对照组。术后24小时、72小时、1周、2周、4周和8周分别取材作组织学检查；1、2、4周取材的部分标本作碱性磷酸酶（ALP）活性测定。结果发现实验组有大量新生软骨和骨形成，随着时间推移，生成量逐渐增多；对照组无软骨和骨形成；此外，实验组ALP活性明显高于对照组（P＜0.001），并且随时间推移而升高。表明β-TCP／rhBMP-2具有良好的骨诱导作用。%To determine the osteoinductive potential of β-TCP／rhBMP-2 composite，the β-TCP／rhBMP-2 composite was obtained by combining Beta-tricalcium phosphate （β-TCP） prepared with recombinant human morphogenetic protein-2 （rhBMP-2）and was implanted into muscle pouches in the thigh of mice. β-TCPalone was implanted on the opposite site as controls.At intervals of 24 and 72 hours and 1，2，4 and 8 weeks after the implantation，the specimens were obtained，and histologic study and alkaline phosphatase assay（1，2，4 weeks）were performed.It was found that there was a large amount of new cartilage and bone formation within the composite，and incereasing with time.Meanwhile，there was no new bone formation where β-TCP alone was implanted.The levels of alkaline phosphatase in the β-TCP／rhBMP-2 implants also were increasing with time and were higher than those in controls （P＜0.001）.The results suggest that β-TCP／rhBMP-2 possesses heterotopic osteoinductive potential.