哮喘发作期患者血清中IL-12、IgE 浓度变化及临床意义

摘要

To evaluate the relationship and the clinical significancebetween the serum IL-12 and IgE in the patients with asthma in attack.Sandwich ELISA was used to detect the IL-12 and IgE level in the sera of 27 cases of patients with athma in attack.SPSS statical software was used to analysis the reletive of IL-12 and IgE.Results showed that the serum IL-12 in 27 cases of patients with asthma in attack was 71.70±81.43pg／ml，it decresed significantly comparing with the health voluteer donor，which is 196.32±94.33pg／ml.To the contrast，serum IgE in patients was 2078.63±611.79pg／ml，which significantly increaed comparing with the normal（327.16±125.28pg／ml）.Linear regression showed that the decrease of IL-12 relate to the increase of IgE（R＝-0.667）.The results suggest that insufficient production of IL-12 imped the differenciation of Th1.Biased Th2 response may be dominat in the immune response of the asthma in attack.%为探讨哮喘发作期患者血清白细胞介素-12（IL-12）与免疫球蛋白E（IgE）的关系及其在哮喘发作中的作用，采用双抗体夹心ELISA方法检测了27例哮喘发作期患者血清中的IL-12和IgE水平，并采用SPSS统计分析软件分析了两者之间的关系。结果显示，27例患者血清中IL-12的平均含量（71.70±81.43pg／ml）显著低于正常对照组（196.32±94.33pg／ml），P＜0.01；IgE含量（2078.63±611.79ng／ml）显著高于对照组（327.16±125.28ng／ml），P＜0.001。统计分析表明，IL-12降低与IgE升高有显著相关性。提示患者体内IL-12产生量不足，可能阻碍了Th1细胞的分化发育，引起了偏离的Th2型的免疫应答，导致超敏反应发生。