维生素A对宫颈癌细胞增殖及凋亡的影响

摘要

目的 观察维生素A对宫颈癌细胞增殖和凋亡的影响.方法 采用MTT法检测5、7.5、10、15 μg/ml维生素A处理后的人宫颈癌Caski细胞的增殖抑制率；采用流式细胞术检测维生素A处理后的Caski、Siha、Hela细胞的细胞周期,荧光酶标仪检测细胞线粒体膜电位；TUNEL法检测维生素A处理后Caski细胞的凋亡情况.结果 5、7.5、10、15 μg/ml维生素A处理24h后的Caski细胞增殖抑制率分别为32.1％±2.6％、47.3％±1.1％、66.4％±2.1％、70.8％±2.7％,处理48 h后分别为38.3％±2.8％、52.6％±3.1％、72.5％±2.4％、80.0％±4.9％,其增殖抑制作用呈时间和浓度依赖性(P均＜0.05)；维生素A处理后,Caski、Siha、Hela细胞G0/G1和G2/M期细胞数量明显减少,S期细胞增多,细胞内线粒体电位分别为2.21±0.29、2.05±0.35、2.38±0.22,与处理前的0.32±0.08、0.24±0.15、0.41±0.06相比,P均＜0.05；维生素A处理后Caski细胞发生凋亡.结论 维生素A可抑制宫颈癌细胞增殖,并诱导其凋亡.%Objective To exploe the effect of vitamin A on cervical cancer cell proliferation and apoptosis. Methods The proliferation inhibition rate of human cervical cancer Caski was analyzed by MTT after treating by 5, 7. 5, 10, 15 μg/ml of vitamin A. Cell cycle of Caski, Siha and Hela treated by vitamin a were detected by flow cytometry. Mitochondri-al membrane potential was examined by fluorescence microplate reader; Apoptosis of Caski cell treated with vitamin A was detected by deoxynucleotidyl TUNEL. Results Cell proliferation inhibition rates of Caski treated by 5,7.5,10,15 μg/ml vitamin A for 24 h were 32. 1% ±2.6%, 47.3% ±1.1%, 66.4% ±2.1%, 70.8% ±2.7%, respectively, after 48 h treatment were 38.3% ±2.8% , 52.6% ±3. 1% , 72.5% ±2.4% , 80.0% ±4.9%. Vitamin A can inhibit cervical cell proliferation in a time and concentration dependent manner (all P < 0.05). The number of Caski, Siha and Hela cells after vitamin A treatment in G0/G1 and G2/M phase significantly reduced, while S phase cells increased, cells were blocked in the Go phase, the mitochondria potential of Caski, Siha and Hela cells was 2. 21 ±0. 29, 2. 05 ±0. 35, 2. 38 ±0. 22, respectively, and pre-treatment was 0. 32 ± 0.08, 0. 24 ± 0. 15, 0.41 ± 0. 06 (all P < 0. 05). Conclusions Vitamin A can inhibit cervical cancer cell proliferation and induced apoptosis.