颞下颌关节紊乱病患者关节液MMP-2、Hyp水平变化及意义

摘要

Objective To investigate the changes and significance of the matrix metalloproteinase-2 ( MMP-2) and hydroxyproline( Hyp) level in synovial fluid of patients with temporomandibular joint disorder ( TMD). Methods Total 94 samples were collected from 105 of TMD patients, which were divided into three groups according to the clinical examina-tion and image analysis: 30 samples of arthritis inflammation patients (inflammation group), 42 samples of internal de-rangement (derangement group), 33 samples of osteoarthritis ( osteoarthritis group), then 20 of healthy volunteers were collected as the control group. The level of MMP-2 and Hyp in TMD synovial fluid was determined using Enzyme-linked im-munosorbent assay. Results The level of MMP-2 and Hyp in the inflammation group, derangement group and osteoarthritis group were significantly higher than those in the control group, the level of MMP-2 and Hyp in osteoarthritis group and derangement group were significantly lower than those in inflammation group ( all P < 0.01). Conclusions The level of MMP-2 and Hyp of TMD patients were discrepant, and the combined detection of two indexs is beneficial to diagnose the degree of pathological lesion of the TMD.%目的 观察颞下颌关节紊乱病(TMD)基质金属蛋白酶-2(MMP-2)、羟脯氨酸(Hyp)水平变化及意义.方法 94例(105侧)TMD患者,临床检查和影像学检查诊断为关节炎症30侧(炎症组)、结构紊乱42侧(紊乱组)和骨关节病33侧(关节病组),另选20例健康体检者作为对照组,采用双抗体夹心酶联免疫法检测各组关节液标本中MMP-2和Hyp的水平.结果 炎症组、紊乱组、关节病组的MMP-2及Hyp水平均明显高于对照组,紊乱组、关节病组的MMP-2及Hyp水平均明显低于炎症组,P均＜0.01.结论 TMD患者关节液中MMP-2和Hyp水平存在差异,两指标联合检测有助于判断TMD病理损害程度.