18β-GA对人高转移卵巢癌HO-8910PM细胞增殖、黏附和侵袭能力的影响

摘要

目的 观察18β-甘草次酸(18β-GA)对人高转移卵巢癌HO-8910PM细胞增殖、黏附和侵袭能力及黏附斑激酶(FAK)、基质金属蛋白酶(MMP)-9表达的影响.方法 将18β-GA作用于HO-8910PM细胞,采用MTT法检测HO-8910PM细胞增殖抑制率；细胞黏附试验检测细胞黏附能力；Transwell chamber法检测细胞侵袭能力；Western blot法检测细胞FAK、MMP-9蛋白表达水平.结果 18p-GA明显抑制高转移卵巢癌细胞HO-8910PM的生长增殖、黏附和侵袭能力；Western blot法检测表明药物能下调FAK、MMP-9蛋白的表达.结论 18β-GA可抑制HO8910PM细胞黏附、侵袭,其机制可能是通过下调FAK、MMP-9蛋白表达而进行的.%Objective To investigate the effects of 18β-glycyrrhetinic acid( 18p-GA)on the proliferation, adhesion, invasion and the expression of FAK, MMP-9 of human highly metastatic ovarian carcinoma HO-8910PM cells. Methods HO-8910PM cells were treated with 180-GA, then MTT assay was used to determine the growth inhibition; Transwell chamber assay was performed to determine the effect on invasion of the cells by 18β-GA; cell adhesion assay was performed to detect the adhesion of HO-8910PM; Western blot analysis was performed to detect the protein expreesion of FAK and MMP-9. Results 18p-GA inhibited the growth, proliferation, adhesion, invasion in cells; 18p-GA also down-regulated the expression of FAK, MMP-9 protein. Conclusion 18β-GA can inhibit the proliferation, adhesion and invasion of HO-8910PM cells, the mechanism may be involved in the reduction of the expression level of FAK, MMP-9.