目的 探讨连翘三萜类化合物达玛-24-烯-3β-乙酰氧基-20s-醇(DM)对人前列腺癌PC-3细胞的增殖抑制及放疗增敏作用.方法 采用流式细胞术检测不同浓度DM液对PC-3细胞周期、凋亡的影响；PC-3细胞接受6-mVX线照射,用克隆形成法检测细胞存活分数、计算放疗增敏比；端粒重复序列扩增法检测DM对PC-3细胞端粒酶活性的抑制作用；实时定量PCR测定DM对PC-3细胞周期相关基因表达的影响.结果 DM可诱导PC-3细胞凋亡,其作用6h后肿瘤细胞端粒酶活性降低,48 h后渐恢复至正常.DM可使细胞周期相关基因p21、TGF-β、Smad3表达增加,Cyclin D1、CDC25A表达降低(p均＜0 05).DM有放疗增敏作用,其放疗增敏比为1.80.结论 DM可诱导PC-3细胞凋亡,抑制其细胞端粒酶活性,调节细胞周期相关基因表达,对前列腺癌PC-3细胞有放疗增敏作用.%Objective To observe the effects of antiproliferation and radiosensitivity on PC-3 cell of prostate cancer induced by Dammar-24-ene-3β-acetate-20s-ol (DM). Methods Cell cycles and apoptoais of PC-3 cell influenced by different concentrations of DM were detected with flow cytometry; the survival fractions of PC-3 cell were studied by colony-formation efficience after 6-mV X ray radiotherapy was administrated on PC-3 cell, and sensitization enhancement ratio was calculated; the inhibitive activities of telomerase of PC-3 cell influenced by DM were analyzed with combining telomeric repeat amplification protocol (TRAP); the expression levels of cell cycle related genes of PC-3 interfered by DM were evaluated using real-time quantitative reverse-transcription PCR. Results DM can induce the apoptosis of PC-3 cell. The activities of telomerase of PC-3 cell may be reduced by DM after 6 hours and then returned to normal level after 48 hours, DM can increase the expression levels of cell cycle related genes p21, TGF-β and Smad3. However, the expression levels of Cyclin Dl and CDC25A were down-regulated by DM. DM had the effect of radiosensilivity. The sensitization enhancement ratio was 1. 80. Conclusions DM may induce the apoptosis and inhibit the activities of telomerase of PC-3 cell and regulate the expression levels of cell cycle related genes. DM has the effect of radiosensitivity on PC-3 cell of prostate cancer.
展开▼
