Objective To observe the immune response and contraceptive effect of guinea pigs injected with the PH-20 coupling body by different method of injection. Methods Sixty female guinea pigs were divided randomly into two groups (rectal injection group and intraperitoneal injection group) , then each group was subdivided into three groups (experimental group 1 , experimental group 2 and control group, 10 female guinea pigs in each group). To observe morphology of the coupling by electron microscopy, to test vaginal secretory immunoglobulin A (sIgA) level by double sandwich ELISA and blood sIgA by radioimmunoassay. PH-20 specific antibody level in blood was detected by competitive ELISA. Results Blood and vaginal secretions slgA and PH-20 antibody levels in rectal injection group and intraperitoneal injection group were both higher than those in control group ( P <0. 05). Blood and vaginal secretions of guinea pig sIgA and PH-20 antibody levels in experimental group 1 (add Freund complete adjuvant) were higher than those in experimental group 2 ( without adding Freund complete adjuvant) (P < 0. 05). The total positive pregnancy rate in rectal injection group was significantly different from intraperitoneal injection group ( P < 0. 05 ). Conclusions The PH-20 coupling body has a high immune activity and can induce significant humoral response, and the rectal injection is superior to the intraperitoneal injection. Freund' s complete adjuvant is able to enhance the effectiveness of two contraceptive injection.%目的 观察以等比方法配制的新型精子表面特异性透明质酸酶(PH-20)疫苗的不同注射方式对豚鼠免疫应答和避孕的影响.方法 60只雌性豚鼠随机分为两大组(直肠注射组和腹腔注射组),每组再分为3个小组(实验1组、实验2组和对照组,每小组各10只),用电镜观察该新型PH -20疫苗的形态,应用双夹心酶联免疫吸附试验( ELISA)检测豚鼠阴道分泌物中分泌型免疫球蛋白A(sIgA)的水平、放射免疫分析法检测血液sIgA的水平、竞争ELISA技术检测血液PH-20特异性抗体的水平.结果 直肠注射组和腹腔注射组豚鼠血液和阴道分泌物中slgA、PH -20抗体含量均较对照组升高(P均＜0.05);实验1组(添加弗氏完全佐剂)豚鼠在各测量时间点血液和阴道分泌物中slgA、PH-20抗体含量均较实验2组(未添加弗氏完全佐剂)高(P均＜0.05).直肠注射组(50％)和腹腔注射组(76.7％)总阳性怀孕率相比有显著性差异(P＜0.05).结论 该新型PH-20疫苗具有较高的免疫活性,能诱导机体产生明显的体液反应,且直肠注射诱导免疫优于腹腔注射.用弗氏完全佐剂均能增强两种注射方式的避孕效果.
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