原发性肝癌患者DNA损伤修复酶基因多态性分析

摘要

Objective To investigate the relationship between genetic polymorphisms of DNA repair genes and hepato cellular carcinoma. Methods Genetic polymorphisms of DNA repair genes had been identified in 150 pateients with hepa tocellular carcinoma( case group) and 150 healthy individuals ( control group) by PCR-RFLP. Results Compared with control group, frequency of wild-type allele of X-ray repair cross complementing group 1 ( XRCC1) Arg399Gln is higher in case group. No significant difference of urinary 8-hydroxy-2-deoxyguanosine (8-OHdG) levels among different polymor phism genotypes in XRCC1 Argl94Tr, Arg280His, Arg399Gln and human 8-oxoguanine DNA glycosylase 1 ( hOGGl ) Ser326Cys. Conclusion Genetic polymorphisms of DNA repair genes are not associated with hepatocarcinogenesis.%目的 探讨DNA损伤修复酶基因多态性与原发性肝癌发生发展的关系.方法 PCR-RFLP法检测150例肝癌患者(肝癌组)和150例健康体检者(对照组)DNA损伤修复酶基因的表型,并进行比较.结果 肝癌组XRCC1 Arg399Gln位点野生型的比率明显低于对照组(P＜0.05)；XRCC1(Arg194Trp、Arg280His)、hOGG1 Ser326Cys位点多态性型别在两组中出现的频率相近(P＞0.05)；各基因位点不同表型者的尿8-羟基脱氧鸟苷(8-OHdG)水平相比,P均＞0.05.结论 DNA修复酶基因多态性与肝癌的发生发展无明确的相关关系.