Hedgehog信号通路在乳腺癌CD+44CD-24细胞中激活

摘要

Objective To explore the effect of Hedgehog signal pathway in the progress of hreast cancer.Methods CD+44 CD-24 cells and non-CD+44 CD-24 cells were selected from cell suspension in the culture system of a serum-free medium by magnetic activated cell sorting system ( MACS) and injected in NOD/SCID mice, respectively.Real-time RT-PCR was employed to detect the expression of Hedgehog signal molecule SMO and GLI1 mRNA in CD+44CD-24 cells and non-CD+44CD-24cells, respectively.Results CD+44CD-24 cells accounted for 2.25％ of hreast cancer cells.The expression levels of SMO and GLI1 mRNA in CD+44CD-24 cells were higher than those in non-CD+44CD-24 cells (P ＜ 0.05 ) .The rate of tumor formation was 100％ ( 3/3 ) in CD+44CD-24 cells contrast to 50％ ( 2/4) in non-CD+44CD-24 cells.One mouse had lung metastasis, one mouse had lymph node and lung metastasis, and one mouse had liver and lymph node metastasis in CD+44CD-24 mice, while no organ metastasis was displayed in non-CD+44 CD-24 mice.Conclusion Hedgehog signal pathway is activated in breast cancer CD+44 CD-24 cells and plays an important role in breast carcinogenesis.%目的 了解Hedgehog信号通路在乳腺癌发生发展中的作用.方法 采用免疫磁珠法从无血清培养的乳腺癌悬浮细胞中分选CD<'+><,44>CD<'-><,24>细胞和非CD<'+><,44>CD<'-><,24>细胞,分别接种于NOD/SCID鼠乳腺脂肪垫内,观察成瘤情况.采用real-time RT-PCR技术检测Hedgehog信号通路分子SMO和GLI1在CD<'+><,44>CD<'-><,24>细胞和非CD<'+><,44>CD<'-><,24>细胞中的表达情况.结果 分选出的CD<'+><,44>CD<'-><,24>细胞约占细胞总数的2.25%,SMO mRNA和GLI1 mRNA在CD<'+><,44>CD<'-><,24>细胞中的表达均高于其在非CD<'+><,44>CD<'-><,24>细胞中的表达(P均<0.05).CD<'+><,44>CD<'-><,24>组乳腺癌发生率为100%(3/3),发生肺转移、淋巴结和肺转移、肝脏和淋巴结转移各1只;非CD<'+><,44>CD<'-><,24>细胞组乳腺癌发生率为50%(2/4),未见有转移发生.结论 在乳腺癌CD<'+><,44>CD<'-><,24>细胞中Hedgehog信号通路分子表达增强,其与乳腺癌的浸润转移关系密切.