沙棘总黄酮对人脐静脉血管内皮损伤细胞增殖及细胞周期的影响

摘要

Objective To investigate the protective effect of the total flavones of Hippophae Rhamnoides L(TFH) on damaged human umhilical vein endothelial cells (CRL-1730). Methods Injured model of CRL-1730 were induced by H2O2 ,and randomly divided into fourgroups ,the normal group were not given any intervention ;the model group was added 250 μmol/L H2O2 ;the TFH groups were alded TFH with the fina1 concentrations of 50,100,200 μg/ml respectively, and added H2O2(dose ditto) 24 hs after training;the Danshen group was added Danshen injection with the final concentration of 2.5 mg/ml, and added H2O2 ( dose ditto ) 24 hs alter training. Cells were intervened for 4 ha and then determined by MTT colorimetric to detect cell viability,the percentage of cell circles were detected by flow cytometry. Results Conpared with the normad group, the cell viability and the percentage d S phase cells decreased,and the percentage d G2/M phase cells increased in the model group;comparel with the model group, the cell viability and the percentage d S phase cells,G2/M phase cells in the TFH group and Danshen group increased, and the percentage of G0/G1 phase cells decreased. Conclusions The TFH has precise protective effect on damag ed HUVECs, it can raise cell proliferation activity and increase ratio d S phase cells.%目的 探讨沙棘总黄酮对损伤人脐静脉血管内皮细胞株(CRL-1730)的保护作用.方法 采用H2O2诱导CRL-1730细胞损伤模型,并将细胞随机分为四组,正常组不予任何干预;模型组加入250 μmol/L H2O2;沙棘组加入沙棘总黄酮终浓度分别为50、100、200 μg/ml,继续培养24 h后加入H2O2(剂量同上);丹参组加入丹参注射液终浓度2.5 mg/ml,继续培养24 h后加入H2O2(剂量同上).细胞干预4 h后用MTT比色法检测各组细胞活性,并用流式细胞仪测定各组细胞周期.结果 与正常组比较,模型组细胞增殖活性下降,S期细胞比率降低,G2/M期细胞比率增加.与模型组比较,沙棘组及丹参组细胞增殖活性升高,C0/C1期细胞比率降低,S期及G2/M期细胞比率均增加.结论 沙棘总黄酮对受损血管内皮细胞有保护作用,可提高细胞增殖活性,增加S期细胞比率.