宫颈鳞癌组织中P-gp、LRP的表达及临床意义

摘要

目的 探讨多药耐药基因蛋白(P-gp)和肺耐药蛋白(LRP)在宫颈鳞癌组织中的表达及指导宫颈癌化疗的意义.方法 采用Real time RT-PCR和MaxVision免疫组化法检测30份宫颈鳞癌组织(宫颈癌组)及30份正常宫颈组织(正常组)中P-gp编码基因(mdr1)、LRP编码基因(lrp)表达量及P-gp、LRP阳性率,并分析mdr1与lrp的相关性,P-gp、LRP之间及与宫颈鳞癌临床病理参数的相关性.结果 宫颈癌组及正常组mdr1 mRNA表达量分别为45.830±0.570、43.567±0.151,lrp mRNA表达量分别为49.989±0.762、45.138±0.550,P-gP阳性表达率分别为83.3%、20%,LRP阳性表达率分别为86.7%、26.7%,组间比较P均＜0.01;mdrl mRNA、Lrp mRNA及P-gp、LRP表达均无相关性(P＞0.05).结论 联合检测P-gp和LRP在宫颈鳞癌组织中的表达,对宫颈鳞癌化疗药物的选择有重要参考价值.%Objective To investigate the expression of P-glycoprotein (P-gp), lung resistance protein (LRP) in the human cervical squmous cancer,and its guiding significance in the chemotherapy of cervical carcinoma. Methods The expression and positive rate of the P-gp coding gene( mdr1 ) ,LRP coding gene(lrp)were examined by Real time RT-PCR and immunohistochemical assay in 30 specimens of cervical squamous cell carcinoma tissue ( cervical cell carcinoma group) and 30 specimens of normal cervical tissue(normal group). The correlation between mdr1 and lrp, P-gp and LRP,the expression of P-gp , LRP and clinicopathology parameters were examined. Results In cervical cell carcinoma group and normal group,the mRNA expression of mdr1 was 45. 830 ± 0. 570,43. 567 ± 0. 151 ,the mRNA expression of lrp was 49. 989 ±0.762,45. 138 ±0.550,the protein positive rate of P-gp was 83.3％ ,20％ ,the protein positive rate of LRP was 86.7％,26.7％ ( all P ＜0.01 ); The expression and positive rate were not significantly correlated between mdr1 mRNA and lrp mRNA,P-gp and LRP(P＞0.05). Conclusions Co-detection of the expression of P-gp and LRP in human cervical squmous cell carcinoma may be useful in selecting anticancer-drugs.