ATG16L1基因多态性与广西壮族人群炎症性肠病的关系

摘要

目的 研究ATG16L1基因单核苷酸多态性(SNP)位点rs2241880与中国广西壮族炎症性肠病(IBD)易感性的关系.方法 分别收集146例中国广西IBD患者[其中溃疡性结肠炎(UC)86例,克罗恩病(CD)60例]与164例正常对照者的肠黏膜组织,用酚-氯仿法提取DNA,采用聚合酶链反应(PCR)扩增ATG16L1基因,PCR产物片段直接进行DNA测序分析,结果在Genbank基因库上进行序列比较.结果 UC、CD患者与正常对照组之间ATG16L1基因SNP位点rs2241880的等位基因多态性无统计学差异(P＞0.05).结论 ATG16L1基因SNP位点rs2241880与广西壮族IBD患者的易感性无关.%Objective To explore the correlation between susceptibility to inflammatory bowel disease (IBD) in Chinese zhuang population of Guangxi province and ATG16L1 gene single nucleotide polymorphiams (SNP) ,rs2241880. Methods The Intestinal tissue of 146 patients of IBD and 164 healthy people were collected. Genomic DNA was extracted from intestinal tissueusing with phenol chloroform methodology. Polymerase chain reaction was used to amplify the ATG16L1 gene, and products were sequenced using the sequencer. The sequenced results were compared to the normal sequence in Genbank. Results No significant differences were noted in the ATG16L1 gene SNP site ra2241880 polymorphisms among the patients with Crohn s disease, ulcerative colitis and the control subjects (P ＞ 0. 05 ). Conclusions The SNP rs2241880 of ATG16L1 are not found to increase the susceptibility of IBD in Chinese zhuang population of Guangxi province.