目的 探讨脆性组氨酸三联体(FHIT)、mutS同种组织蛋白2(MSH2)及p21蛋白表达在肺癌发生、发展中的意义.方法 应用免疫组化SP法检测89份原发性肺癌组织(原发癌组)、12份淋巴结转移性肺癌组织(转移癌组)、12份肺癌癌前病变组织(癌前变组)及10份正常肺组织(对照组)中FHIT、MSH2及p21蛋白表达情况,分析各指标间相关性及其与肺癌临床病理参数的关系.结果 与对照组比较,FHIT、MSH2及p21蛋白在其他三组中表达均下调(P﹤0.05);FHIT、MSH2蛋白表达与肺癌组织学类型、分化程度相关,p21蛋白表达与肺癌分化程度、临床分期及有无淋巴结转移有关(P均﹤0.05); FHIT和MSH2蛋白在原发癌组表达呈显著正相关(P ＜0.01),但均与p21蛋白表达无明显相关.结论 FHIT、MSH2及p21蛋白与肺癌发生、发展有关,联合检测三项指标可为临床预测疾病进展提供依据.%Objective To investigate the roles of fragile histidine triad gene (FHIT),mutS homolog 2(MSH2) and p21 protein in the genesis and progross expression of lung cancer. Methods The expression of FHIT,MSH2 and p21 protein were detected by SP immunohistochemistry method , in tissues of 89 primary lung cancer( primary cancer group), 12 lymph node metastasis of lung cancer( metastatic carcinoma group ), 12 precancerous lesion (precancerous lesion group) and 10 normal control( control group), the relationship between the indexes and clinicopathological parameters were analyzed. Results Compared with control group, the expression of FHIT,MSH2 and p21 protein decreased in the other three groups(P ＜ 0.05) ;The expression of FH1T and MSH2 protein was related to tumor histologicol types and degree of cell differentiation ,the expression of p21 protein was related to the differentiation degrees,clinical stages and lymph node metastasis ( P ＜ 0.05) ;There was significantly positive correlation between FHIT and MSH2 protein ( P ＜0.01 ), but not obviously correlated with p21 protein. Conclusion The expression of FHIT ,MSH2 and p21 protein was related to the genesis and progress of lung cancer,joint detection of the three indexes may be useful to predict the development of lung cancer.
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