人子宫颈癌组织PD-L1表达及其与HPV16E6/E7的相关性

摘要

Objective To investigate the role of programmed death 1 ligand 1 ( PD-L1) in the occurrence and development of cervical carcinoma. Methods The protein expression of PD-L1, Human papilloma virus 16 type E6 / E7 ( HPV16E6/E7) were examined in 40 samples of cervical carcinoma (cervical carcinoma group) ,25 samples of cervical in-traepithelial neoplasma ( CIN group ) and 8 samples of normal cervical tissue ( control group) by immunohistochemical SABC method and RT-PCR,the correlation between the two indexes was analyzed. Results PD-L1 and HPV16E6/E7 expressed in the cervical carcinoma group and CIN group,and the level in the former were significantly higer (P< 0. 01) ; there was a significant positive correlation between PD-L1 and HPV16E6/E7,r = 0.531, P<0.01. Conclusions PD-L1 can promote the development of cervical carcinoma.%目的 探讨PD-L1在宫颈癌发生、发展中的作用.方法 采用免疫组化SABC法及RT-PCR法分别检测40份宫颈癌组织(宫颈癌组)、25份宫颈上皮内瘤变(CIN)Ⅱ、Ⅲ级组织(CIN组)、8份正常宫颈组织(正常组)中程序性死亡配体1( PD-L1)、HPV16E6/E7(人乳头瘤病毒16型E6/E7)表达并分析二者的相关性.结果 宫颈癌组、CIN组均有PD-L1、HPV16E6/E7表达,宫颈癌组表达明显高于CIN组(P＜0.01)；PD-L1与HPV16E6/E7表达呈正相关,r =0.531,P＜0.01.正常组未见二者表达.结论 PD-L1在人子宫颈癌中发生、发展中起促进作用.