Objective To investigate the prevention and treatment effects of quercetin on pulmonary arterial hypertension ( PAH) . Methods Forty male Sprague-Dawley (SD) rats were randomly divided into four groups with ten rats in each group: model group, quercetin-prevention group (P group) , quercetin-treatment group (T group) and control group. The former three groups were made PAH by the induction of monocrotaline (MCT) , the control group was given the same amount of saline. The model group and control group received saline 2 mL/d by gavage after the molding, the P group and T group received quercetin 100 mg/( kg · d ) by gavage at the same time of molding or 20 ds after, and both for 20ds. The pulmonary arterial pressure (mPAP) of each groups was measured, Percentage of wall thickness(WT% ) and Percentage of wall area( WA% ) of pulmonary arterioles were evaluated; the expression of Bcl-2 in pulmonary arterial smooth muscle was observed by immunohistochemistry staining and western blot. Results Compared with the C group、P group and T group, The mPAP、 WT% 、WA% and Bcl-2% in MCT group increased significantly (P <0. 05). The expressions of Bcl-2 in MCT group also increased significantly (P < 0. 05) than other groups. Conclusions Quercetin has the role of prevention and treatment on the MCT-induced PAH in rats , its mechanism maybe due to reverse pulmonary vascular remodeling through down-regulation of Bcl-2 and promote apoptosis in smooth muscle cells.%目的 探讨槲皮素对肺动脉高压的预防及治疗作用.方法 将成年雄性SD大鼠40只随机分为模型组、预防组、治疗组及对照组各10只.前三组采用野百合碱(MCT)诱导制作肺动脉高压模型,对照组注射等量生理盐水.制模后模型组及对照组予生理盐水2 mL/d灌胃,预防组制模同时、治疗组制模20 d予槲皮素100 mg/( kg·d)灌胃,均为20 d.干预后测定各组平均肺动脉压力(mPAP)、肺小动脉管壁厚度(WT)占动脉外径(ED)的百分比(WT％)及管壁面积(WA)占血管总面积的百分比(WA％)；采用免疫组化和Western blot法检测肺小动脉平滑肌细胞Bcl-2表达.结果 与对照组、预防组和治疗组比较,模型组mPAP、WT％、WA％和Bcl-2％显著升高(P＜0.05),Bcl-2的表达显著增多(P＜0.05).结论 槲皮素对MCT诱导的大鼠肺动脉高压有预防和治疗作用,其机理可能是通过下调肺血管中小动脉平滑肌细胞Bcl-2表达,促进细胞凋亡,改善肺血管重构.
展开▼
