膀胱尿路上皮癌及正常膀胱组织microRNA21、205表达研究

摘要

目的 探讨microRNAs (miRNAs)在膀胱尿路上皮癌与正常膀胱组织中的表达差异性及意义.方法 采用基于2-△△cT的实时定量PCR(Real-time PCR)方法 检测50例膀胱尿路上皮癌与21例正常膀胱组织中miR-21及miR-205的表达情况.结果 与正常组织相比,癌组织miR-21表达上调(P＜0.01),miR-205表达下调(P＜o.o1).癌组织中miR-21与miR-205表达量的比值约3.6倍于正常组织中表达量的比值.结论 miR-21高表达、miR-205低表达可能参与膀胱尿路上皮癌的发生、发展.%Objective To detect the expression of miR-21 and miR-205 in urothelial cell carcinoma of bladder and normal bladder mucosa and explore the relationship between microRNAs (miRNAs) and urothelial cell carcinoma of bladder. Methods 2△△CT Real-time PCR method was used for quantitative analysis of the expression of miR-21 and miR-205 in 50 urothelial cell carcinoma of bladder and 21 normal bladder tissues. Results Compared with normal bladder mucosa, the urothelial cell carcinoma of bladder tissues showed significant miR-21 over-expression (P<0. 01) and miR-205 down-regulation (P <0. 01). Cancer tissue showed miR-21: miR-205 ratio at least 3.6-fold higher than the quantitative ratio obtained from normal bladder tissue. Conclusion The over expression of miR-21 and lower expression of miR-205 may participate in recurrence urothelial cell carcinoma of bladder.