Objective To establish the immortalized B lymphoblastoid cell lines (B-LCL) from patients with primary immunodeficiency diseases (PIDD) in vitro, so to provide related resources for further study of PIUD. Methods The peripheral blood mononuclear cells (PBMCs) were isolated from the patients with PIDD by Ficoll density gradient centrifuga-tion method, then were incubated with Epslein-Barr virus ( EBV) in the presence of cyclosporin A ( CSA) to establish B-LCL by inhibiting the activity of T lymphocytes. Morphology of the transformed cells was observed under inverted microscope. Karyotype analysis of PBMCs and B-LCL was performed. Results The cellular volume of those transformed cells was enlarged and there was increasing development of cell aggregates; the immortalized B-LCLs could be cultured and propagated for a long time in vitro with no significant change in karyotype. Conclusion Immortalized B-LCL from patients with PIDD is successfully established in this study, which provides a basis for the etiological research, diagnosis and treatment of PIDD.%目的 体外建立原发性免疫缺陷病(PIDD)患者永生化B淋巴母细胞系(B-LCL),为进一步开展PIDD相关研究提供资源.方法 自PIDD患者外周血中分离单个核细胞,采用EB病毒感染并加入环胞菌素A抑制T淋巴细胞活性的方法建立B-LCL,7d左右倒置显微镜下观察转化细胞的形态,并进行染色体核型分析.结果 转化后的B淋巴母细胞体积增大,增生的淋巴细胞多聚集成团；已建立的永生化细胞能够成功冻存、复苏及传代；转化前后细胞染色体核型无明显改变.结论 本实验成功建立了PIDD患者永生化的B-LCL,为进一步开展PIDD的病因学研究及诊断、治疗奠定了基础.
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