牛磺酸对急性重型颅脑创伤大鼠脑血流的影响

摘要

目的 探讨急性重型颅脑创伤(TBI)大鼠大脑皮质脑血流(CBF)变化以及牛磺酸(Tau)的治疗效果.方法 选择SD大鼠40只,随机分为假手术组(Sham组)、脑创伤组(TBI组)、Tau低剂量组(100 mg/kg)、Tau高剂量组(200 mg/kg)各10只.采用液压打击法在大鼠大脑左侧制作TBI模型,Sham组只开骨窗.Tau组伤后立即尾静脉注射相应剂量Tau,TBI组给予相同量生理盐水.采用激光多普勒血流仪检测各组TBI前及TBI后30 min、24h两侧大脑皮质CBF变化.结果 各组TBI前CBF比较均无统计学意义.左脑:模型组TBI后30 min、24 h CBF较Sham组均明显减低(P均＜0.05)；TBI后30 min,与TBI组比较,Tau低剂量组CBF无明显变化,Tau高剂量组显著升高(P＜0.01)；TBI后24h,Tau高、低剂量组CBF均明显高于伤后30 min(P＜0.05).右脑:与TBI组比较,Tau低剂量组CBF在TBI后30 min无明显变化,TBI后24h显著升高(P＜0.05)；Tau高剂量组TBI后30 min、24 h CBF均明显升高(P均＜0.05)；Tau高、低剂量组CBF在各时段均无统计学意义.左右脑:TBI组、Tau低剂量组和Tau高剂量组左脑CBF TBI后30 min明显低于右脑；TBI后24h后左右脑的CBF比较无统计学意义.结论 Tau治疗有助于改善TBI大鼠脑创伤后CBF状况,其改善程度与用药时间、剂量呈正比.%Objective To investigate the changes of rat cerebral cortex blood flow (CBF)after acute severe traumatic brain injury (TBI) and the effects treated by taurine (Tau). Methods Forty SD rats were randomly divided into sham op-eration group (group sham), brain trauma group (group TBI), Tau low dose group (100 mg/ kg) , high dose of Tau group (200 ing/kg) equally. TBI models were made in left brain of rats by hydraulic shock, while sham group only opened bone window. Rats in group Tau were given Tau immediately after injury injected via tail vein; And group sham, group TBI was given the same amount of physiological saline. Laser doppler flowmetry was used to monitor the cerebral cortex CBF of each group in the time before trauma, 30 min after injury and 24 h before puting to death. Results There was no statistical sig-nificance of CBF in each group before injury; CBF in group TBI was significantly lower than that in group sham on points 30 min or 24 h after injury (P<0.05). The left brain: compared with TBI group, CBF in group Tau low dose showed no sig-nificant difference, whereas high dose of Tau group CBF significantly increased (P <0.01) ; CBF on 24 h after injury was significantly higher than that on 30 min after injury in group Tau high or low dose (P < 0.05 ). The right brain: compared with group TBI, CBF in Tau low dose group was no significant difference on 30 min after injury, but increased obviously af-ter 24 h (P < 0.05 ) ; CBF in Tau high dose group increased significantly on 30 min and 24 h after injury (P < 0. 05 ) ; there was no statistical significance overall between group Tau high and low dose. Left and right brain; CBF TBI of left brain was significantly lower than that of right brain on 30 min after injury in all three groups, and there was no statistical significance between each group on 24 h after injury. Conclusions Tau treatment helps improve CBF after TBI, and its improvement is positively related to administration time, dosage.