人胃癌细胞株MGC-803侧群细胞的分选与生物学特征

摘要

目的 从人胃癌细胞株MGC-803中分离侧群(SP)细胞,研究其生物学特征.方法 利用流式细胞分选术(FACS)从MGC-803细胞株中分选出SP细胞和非SP细胞亚群进行培养,采用克隆形成实验比较两组亚群细胞的体外增殖能力,NOD/SCID鼠成瘤实验检测两组亚群细胞体内成瘤能力.结果 FACS结果显示,细胞株MGC-803中SP细胞亚群占总细胞的0.3％ ～ 1.2％;SP细胞体外克隆形成率为(0.862±0.050)％,非SP细胞体外克隆形成率为(0.325 ±0.207)％,两者比较P＜0.05;SP细胞最低成瘤数量是1×103/只,非SP细胞为5×103/只.结论 人胃癌细胞株MGC-803中存在SP细胞,其生物学特性与干细胞基本符合.%Objective To investigate isolation and biologic characteristics of the side population(SP) in human gastric cancer cell lines MGC-803. Methods Fluoresence activated cell sorting was used to sort SP and NSP cells from MGC-803 s. Tumorigenicity of the two subpopulations was observed by sphere forming assay. Proliferation was determined by subcutaneous tumor formation in NOD/SCID mice. Results The percent of SP cells was 0. 3% -1.2%. Clone formation efficiency of SP cells and NSP cells was(0. 862 ± 0.050) % and(0. 325 ± 0.207) % respectively, there were significant differences ( P < 0.05). In vivo, the tumorigenic ability of SP cells was higher than that of NSP cells. Tumor formed only by 1 x 103 SP cells in nude mice, but 5 x 103 NSP cells. Conclusion Human gastric cancer cell lines MGC-803 contained some SP cells with stem cell properties.